Journal of Patient Safety and Infection Control

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 5  |  Issue : 2  |  Page : 62--68

Molecular characterisation of beta-lactamases-producing Enterobacteriaceae members from critical care patients


Richa Gupta1, Abida Malik1, Meher Rizvi1, Syed Moied Ahmed2 
1 Department of Microbiology, JNMC, AMU, Aligarh, Uttar Pradesh, India
2 Department of Anaesthesiology, JNMC, AMU, Aligarh, Uttar Pradesh, India

Correspondence Address:
Dr. Richa Gupta
Department of Microbiology, JNMC, AMU, Aligarh, Uttar Pradesh
India

Background: This study was done to assess the prevalence of beta-lactamases and biofilm production in general and blaCTX-M, blaTEM, blaSHV and blaAmpC in particular amongst Enterobacteriaceae members in Intensive Care Unit (ICU) patients. Materials and Methods: Samples were collected aseptically from 210 ICU patients from February 2012 to December 2015. Culture, identification, antimicrobial susceptibility, extended-spectrum beta-lactamases (ESBLs), AmpC, metallo-beta-lactamase (MBL) and biofilm detection were done according to the standard protocol. Polymerase chain reaction analysis for beta-lactamase genes of the family CTX-M, TEM, SHV and AmpC was carried out. Results: Amongst 200 pathogens, most commonly isolated Enterobacteriaceae member was Escherichia coli (2 [26%]), Klebsiella pneumonia (37 [18.5%]), Klebsiella oxytoca (12 [6%]), followed by Citrobacter species (33 [16.5%]) and Serratia species (15 [8%]). E. coli (29 [55.7%]) was most commonly associated with urinary tract infection; however, the frequency of K. pneumoniae (18 [48.6%]), K. oxytoca (5 [41.6%]) and Citrobacter koseri (20 [33%]) was higher in lower respiratory tract, bloodstream and surgical site infections, respectively. Phenotypically, prevalence of ESBL, AmpC and MBL amongst Enterobacteriaceae members was 50.2%, 36.6% and 12.3%, respectively. However, blaCTX-M and blaAmpC genes were detected in 48% and 32.4% of members, respectively. BlaTEM and blaSHV were not detected in any of the isolates. The average hospital stay of ICU patients was 21 days and was associated with 48.5% mortality. Conclusion: There is a great need for informed antibiotic treatment guided by not only routine antimicrobial susceptibility but also by knowledge of ESBL, AmpC and MBL status of the isolate.


How to cite this article:
Gupta R, Malik A, Rizvi M, Ahmed SM. Molecular characterisation of beta-lactamases-producing Enterobacteriaceae members from critical care patients.J Patient Saf Infect Control 2017;5:62-68


How to cite this URL:
Gupta R, Malik A, Rizvi M, Ahmed SM. Molecular characterisation of beta-lactamases-producing Enterobacteriaceae members from critical care patients. J Patient Saf Infect Control [serial online] 2017 [cited 2019 Jul 23 ];5:62-68
Available from: http://www.jpsiconline.com/article.asp?issn=2214-207X;year=2017;volume=5;issue=2;spage=62;epage=68;aulast=Gupta;type=0